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KMID : 1123920100240040616
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Korean Journal of Oriental Physiology and Pathology
2010 Volume.24 No. 4 p.616 ~ p.623
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Stimulation of the Immune Response by Herbal Formulas for Wind-Cold and Heat Pain Symptom
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Jung Da-Young
Ha Hye-Kyung
Lee Ho-Young
Lee Jin-Ah
Lee Jun-Kyoung
Huang Dae-Sun
Shin Hyeun-Kyoo
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Abstract
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Three herbal formulas (Bangpungtongsung-san, Ohyaksungi-san, and Ojeok-san) for wind-cold and heat pain symptom were applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in murine macrophage cell line (RAW 264.7) and ovalbumin (OVA)-immunized mice. This study was carried out in nitric oxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes according to three herbal formulas. C57BL/6 mice were immunized intraperitonially with OVA/aluminium (/mouse) on day 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, lgG1, and total IgM classes) in plasma were measured. Ohyaksungi-san increased NO synthesis in RAW 264.7 cells. Ojeok-san and Ohyaksungi-san significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<0.001). Three herbal formulas for wind-cold and heat pain symptom also significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group. These results suggested that three herbal formulas for wind-cold and heat pain symptom could be used as stimulator of immune response.
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KEYWORD
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Bangpungtongsung-san (Fangfengtongsheng-san), Ohyaksungi-san (Wuyaoshunqi-san), Ojeok-san (Wuji-san), Immune response
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